- Welcome, everyone to
We

 

dnesday Nite at the Lab.

 

I'm Tom Zinnen.

 

I work here at the UW-Madison
Biotechnology Center.

 

I also work for UW-Extension
Cooperative Extension,

 

and on behalf of those folks
and our other co-organizers,

 

Wisconsin Public Television,

 

the Wisconsin Alumni Association

 

and the
UW-Madison Science Alliance.

 

Thanks again for coming to
We

 

dnesday Nite at the Lab.

 

We do this every Wednesday
night, 50 times a year.

 

Tonight, it's my pleasure to
introduce to you Nick Smith.

 

He is the brand new
enologist here at

 

UW-Madison in the
Department of Food Science.

 

He was born in Lionel
Lakes, Minnesota,

 

and went to the University
of Minnesota for his

 

undergraduate on the
Minneapolis campus.

 

Then he got his enology training
at Oregon State University,

 

worked for a couple of
years in California,

 

came back to the Twin Cities

 

to get a master's degree
in food science

 

on the St. Paul campus,
and then in April came

 

to Madison to be,
as far as I know,

 

the first dedicated
enologist here at UW-Madison.

 

I think it's a great
time to be thinking about

 

grapes and wine
here in Wisconsin.

 

Please join me in
welcoming Nick Smith

 

to Wednesday Nite at the Lab.

 

(Applause)

 

- Thanks, Tom.
Good evening.

 

Happy you all could
come out tonight

 

and talk about some
Wisconsin wine.

 

Occasionally I have a
little trouble switching

 

to Wisconsin since for so
many years I said Minnesota.

 

(Laughter)

 

Forgive me if I make a
little slip up there.

 

As Tom mentioned, I
am the first dedicated

 

enologist here at
University of Wisconsin,

 

part of the brand new
Fermentation Sciences program,

 

which I will introduce
shortly here.

 

Kind of a rundown of what
we'll talk about tonight.

 

I'm going to introduce
myself a little bit, although

 

Tom did a very good
job of that already,

 

as well as the new program
and the new project

 

that we have going here at
the University of Wisconsin.

 

We'll talk a little bit
about Wisconsin grape

 

and wine history, move on
to some industry statistics,

 

and then kind of go
into discussions of
cold climate grapes,

 

what makes them
unique, how those

 

uniquenesses impact
wine production

 

and what I study and
research and work with the

 

industry on improving,
talking about a bit about

 

quality and what
that really means,

 

and then talking about
the research that we have

 

going on here at the
University of Wisconsin,

 

both of what I'm doing
and a bit of what the

 

horticulture department
is doing as well

 

as the genetics department
here, right above us actually.

 

So it's a brand new position.

 

I started March 28th.

 

So I've only been
here a few months.

 

So it's part of the
Fermentation Sciences program.

 

Dr. Jim Steele is the
chair of that program.

 

We're getting that
started so I'm basically

 

the first employee of
that program as well.

 

So they're doing a lot
of research on beer

 

and wine production up there.

 

What makes this interesting
is this position

 

was started by an initiative
from the industry itself.

 

It is not a university based,

 

they did not sponsor
it initially.

 

The Wisconsin Vintners
Association, along

 

with the Wisconsin
Winery Association,

 

the Wisconsin Grape
Growers Association,

 

got together, teamed up,

 

and wrote a grant to
fund this position.

 

And we got funding
for several years

 

to get the position started.

 

What's fascinating
about this from my

 

perspective, having
worked in other states in

 

the industry, is that
the first one on there

 

is the Amateur Wine
Association out of Milwaukee.

 

So to have an amateur
wine association be one

 

of the biggest supporters
and proponents of

 

our program I think
is pretty amazing.

 

And they've been very
helpful in getting

 

this all started and organized.

 

So the position itself
main focus is on

 

improving the quality
of Wisconsin wine.

 

So my main objectives
are to go out,

 

meet with wineries,
address quality concerns,

 

do analysis, as well as
formulate research to

 

help improve the overall quality
of Wisconsin wine and grapes.

 

My background, as Tom mentioned,

 

is I originally got my
undergraduate degree in

 

finance and marketing
from the University of

 

Minnesota's Carlson
School of Management,

 

ended up continuing on in
food science for a while.

 

During that time, I spent
a year at Oregon State

 

studying food science,
finished up that year,

 

and the headed south
into California where

 

I started an
internship a year ago,

 

10 years ago in August of 2015.

 

That's when I kind of
got full on into wine.

 

So I worked at a facility
that used to be owned

 

by Ste. Michelle Wine Estates
out of Hopland, California.

 

Finished my internship
there and got a job,

 

position at Beringer
Vineyards working as a

 

wine chemist doing
lots of lab analysis.

 

Before I actually went out west,

 

I actually did research
at the University of

 

Minnesota for the
enologist who was there.

 

She informed me that
there was a position

 

opening up at the
University of Minnesota,

 

applied for that position,
and eventually moved

 

back to the University
of Minnesota about 2006

 

to do research wine
production there.

 

I was the research winemaker

 

and wine analyst for
the breeding program.

 

Briefly between here
and University of

 

Minnesota I did go
back and do commercial

 

wine production and
cider production at Four

 

Daughters Winery and Vineyard
down in Rochester, Minnesota.

 

And then when this
position opened up,

 

I applied and accepted and
shortly thereafter moved here.

 

So it was a very
quick succession.

 

Before we talk about
Wisconsin wine history,

 

we're all here tonight
so I'm assuming

 

most of you are wine consumers.

 

Is that correct?
(Laughter)

 

All right, so, next
question is, how many of you

 

are actually avid consumers
of Wisconsin wine?

 

A very good show of hands.

 

Great. I ask those
questions in Minnesota

 

and don't always get
the same response.

 

So that's good.

 

They've been growing
grapes for quite a long

 

time on some level in
Wisconsin since the

 

1840s when Agoston
started the vineyard not

 

far north of here,
what eventually would
become Wollersheim.

 

And the first commercial winery
in the state started in 1867.

 

So we're going on
close to 50 years

 

of having commercial wine

 

production in the
state of Wisconsin.

 

Wollersheim started in 1972.

 

They're prominent
because they are

 

the largest winery
here in Wisconsin.

 

There's been several
viticulture areas that have

 

been named and
established in Wisconsin

 

viticulture areas are
areas recognized by the

 

federal government for
having very specific

 

wine growing, grape
growing characteristics.

 

So it's good for marketing
and differentiation of

 

the wineries and grapes
grown in those areas.

 

University of Wisconsin
didn't really start

 

getting into grape
research probably around

 

2000, 2005, 2008
when they started

 

establishing vineyards
at the agricultural

 

research sites around the state,

 

one of which is over
here in west Madison.

 

And then in 2015, just
shortly not long ago,

 

that's when I came on board.

 

So that's kind of the
history of Wisconsin wine.

 

A little bit of statistics.

 

Currently, there are
actually about 110 wineries

 

licensed in the
state of Wisconsin.

 

So you can see from
several years ago, that's

 

quite an increase
of over 20 wineries.

 

We're growing roughly five to
10 wineries every single year.

 

That's been consistent for
the last five or 10 years.

 

Up to probably 700-plus
acres of grapes.

 

I would say probably
more than that.

 

It's a little hard to get
a good estimate since a

 

lot of small growers don't
report how many acres they have.

 

You can see in the last
13 years, they've more

 

than doubled the size and number

 

of grapes grown
here in Wisconsin.

 

The five largest wineries
are Wollersheim, Door

 

44, Parallel 44, which
are the same business,

 

Danzinger Vineyards,
Door Peninsula and Elmaro.

 

A couple wineries that
I've kind of gotten into

 

recently are Dancing
Dragonfly and Villa Bellezza.

 

I think those will
probably move into the top

 

five here pretty soon.

 

We should be proud of
some of our wineries,

 

particularly Wollersheim Winery.

 

It's one of the largest
independent held

 

wineries outside of California,

 

including Oregon and Washington.

 

So I think it's about 13th.

 

They're producing 100,000
cases, roughly, every year.

 

We'll talk a bit about
cold climate grapes.

 

Some of you are
probably familiar

 

with these particular varieties.

 

Worked a lot with
these varieties

 

when I was at the
University of Minnesota.

 

Marquette had just gotten
introduced when I started there.

 

So I'm probably one of
the few people who has

 

the most experience of
actually producing that grape.

 

So the cold climate
grapes, these hybrids,

 

they're an interspecific
cross between either

 

Vitis labrusca or
Vitis riparia and

 

the typical wine grapes
of Vitis vinifera.

 

Vitis vinifera are
your Chardonnays,

 

your Merlots, and
your Cabernets.

 

Vitis riparia is also
known as river grapes since

 

that's where it
predominately grows are in

 

rivers along the riverbanks.

 

Very small berries, small
clusters, dark juice,

 

high acid, very high
sugar, herbaceous flavor.

 

I talk about these
characteristics because a lot

 

of this genetics get
carried over into our other

 

varieties that we're
trying to grow now.

 

These are the type of
things that we have to

 

learn how to handle when we
try to make these into wine.

 

On the other side there's
labrusca varieties

 

which tend to be a little
bit lower in sugar, but

 

they have a very
specific flavor profile.

 

The University of Minnesota
has relied a lot on

 

Vitis riparia for its
cold climate genetics.

 

Whereas, Elmer Swenson,
who I'll mention again here

 

shortly, since he's a Wisconsin
based grape breeder, he

 

used a lot of labrusca based.

 

When we think of hybrids we
think of them in kind of one

 

general camp, but there's
really two sort of separate

 

angles that they can take.

 

And the challenges that they

 

present aren't
quite all the same.

 

So you can see the Vitis
riparia is grown pretty much

 

native throughout
the Upper Midwest

 

and Canada and down
into even Texas.

 

You can see from
this picture maybe

 

just how tiny and scraggly
those grape clusters are.

 

So they're kind of
hard to deal with.

 

Very tiny berries.
Lots of pigment.

 

It takes a lot of picking to get

 

any real production
out of those.

 

So, in terms of breeding and
varieties you might see here

 

in the Wisconsin market,
MarÈchal Foch and LÈon Millot...

 

are things that are grown
quite a bit here in the

 

southeastern part of Wisconsin.

 

Wollersheim grows
a lot of these.

 

Botham Vineyard grows both
of these pretty extensively.

 

And they've been around
since 1910 or in the 1910s

 

when a French breeder
named Eugene Kuhlmann

 

produced those varieties,

 

and they've been very
popular ever since.

 

They're kind of the basis for
cold climate wine production.

 

And in Minnesota and north
and west of here, they don't

 

grow as well as they do here.

 

So other breeders, including
the University of Minnesota,

 

have been working on
trying to improve on those

 

and create other
varieties that are better

 

suited for these climates.

 

The University of
Minnesota has been working

 

on grapes for a very
long period of time,

 

started breeding
roughly in 1905,

 

and started coming out with
their first round of grapes

 

that they released
in about 1944.

 

I highlighted Bluebell
there since that's one

 

you occasionally do see
wineries producing in the wine.

 

I don't know if the other three
really even exist anymore.

 

I think they might
actually be extinct.

 

But the initial
emphasis of the program

 

was on table grapes,
juice, jelly production.

 

It wasn't until about
the '70s or '80s when

 

they actually shift
focus and put more energy

 

and effort into actually
wine grape production.

 

Their first wine grape was
released in '96 called--

 

which many of you who
have been to wineries

 

around the state
have probably seen.

 

They're actually hinting that
they'll release a new variety,

 

at least when I was still
working there they hinted at it.

 

I don't know what the
current status of that is,

 

but hopefully they'll
have that out soon.

 

On top of the
university breeders,

 

there's been a lot
of private breeders.

 

There's a lot of people with an

 

interest in growing
these varieties.

 

So way back even in the 1870s,

 

Lewis Swelter created
numerous varieties,

 

but one of his
biggest ones was Beta,

 

which would have been
one of the most popular

 

varieties grown in the Upper
Midwest prior to Prohibition

 

and was actually
pretty widely grown

 

in both Minnesota and Iowa.

 

David MacGregor is
a private breeder.

 

He came up with Petite Amie,
which isn't necessarily

 

very popular here in Wisconsin,

 

but you do find some
of it in Minnesota.

 

After that, of course,
is Tom Plocher.

 

He created the Petite Pearl,
which is becoming a popular

 

grape grown, or a very
interested variety,

 

here in Wisconsin.

 

It's a relatively new
variety so we don't know

 

a great deal about it.

 

We got some of those grapes

 

from the research
center this year.

 

So we'll get some new
information on those.

 

And he's planning to
breed at least a couple

 

new varieties here this year.

 

One of the other
major universities

 

that does grape breeding
is also New York.

 

I mention them mostly because
of varieties like Traminette.

 

You might see those
in local wineries.

 

Traminette is not cold
hardy enough for Wisconsin,

 

but they do grow in
Illinois and Arkansas.

 

So you occasionally do see them

 

in tasting rooms
around Wisconsin.

 

I can't talk about
Wisconsin grape

 

breeding without mentioning
Elmer Swenson, who many consider

 

the godfather of grape breeding,

 

especially the
Wisconsin supporters.

 

It should be noted, though,

 

that he did work for the
University of Minnesota

 

for about 10 years,
and while he was

 

there they joint
released two varieties.

 

Edelweiss, which is a
common grape variety that

 

is turned into wine,
particularly here and Nebraska,

 

as well as Swenson Red.

 

And he created a whole slew
of other grape varieties.

 

He also produced and released
St. Pepin, a popular one,

 

and Brianna around
here and a few of those

 

other varieties
you might see when

 

you're out and about
in tasting rooms.

 

So, what's the general
differences between
these varieties?

 

Well, one is color.

 

In terms of red
grapes, the color

 

intensity and the pigmentation

 

is very intense compared to,
say, your average vinifera.

 

One of the things
that they have besides

 

pigment in the skins is
also their pigmented pulp.

 

Cabernet and Pinot
noir have a clear pulp.

 

Our varieties actually
have a pigmented pulp.

 

So when you press
these things out,

 

they're actually very dark
right from the beginning.

 

And the white grapes, they
have a bit of a yellow tint,

 

but they also can brown a bit.

 

So there's a high
polyphenol oxidase activity.

 

If you ever cut open an
apple or a banana starts

 

to turn brown, so those
enzymes also exist in grapes.

 

The hybrids seem to
have a lot more of those

 

then many of the vinifera.

 

One thing you'll
definitely notice

 

if you try cold climate wines is

 

that they tend to be
a lot more acidic.

 

Quite a bit more
than Vitis vinifera.

 

Titratable acidity,
11 grams per liter

 

to 15 to 17 grams per liter.

 

A majority of that is
actually malic acid,

 

where vinifera is
typically 50/50.

 

It's got an even split
of tartaric to malic,

 

where some of these
varieties, like La Crescent,

 

can be almost 75% malic
acid, which is important when

 

we discuss later how we
manage the acid in the winery.

 

So just kind of
a general summary

 

of some titratable acidities

 

just to give you an idea
that Frontenac is roughly two

 

to three times more acidic than
your average red table wine.

 

Sometimes three times as much.

 

So these are some of
the challenges that
we have to embrace

 

and work with when we're making
wines from these varieties.

 

But there are other
varieties out there,

 

other styles of wine out there

 

that could fit those quite well.

 

So, sparkling wine is
probably a good use

 

for some of these varieties.

 

One other difference is the
amount of tannin in the variety,

 

especially red grapes.

 

Very little tannins
in the finished wine.

 

And it's almost
completely opposite.

 

So, vinifera have a
great deal of tannins

 

and not nearly as much
anthocyanin content,

 

where the cold climate
varieties have a tremendous

 

amount of anthocyanins
and not a lot of tannins.

 

So theirs is kind of reversed.

 

A lot of people
would like to see

 

more tannins in their varieties.

 

I kind of prefer
the fact that we

 

don't have a lot
of tannin in that.

 

If we had high
tannin and high acid,

 

it'd be a very difficult
wine to work with,

 

even more than it already is.

 

Acid tends to increase
the perception of tannins,

 

as well as tannins increasing
the perception of acid.

 

So having both of those
would be a bit challenging.

 

The sugar content can vary a
bit depending on the variety,

 

it's parentage, and
where it's grown.

 

One of the challenges I
had when I was in Minnesota

 

is that the varieties
accumulated a lot of sugar.

 

So it could be a
very alcoholic wine

 

or put a lot of stress on these.

 

Don't seem to have that
issue down here in Wisconsin,

 

in this part of
Wisconsin as much,

 

but those are some of
the issues you can have.

 

The fact that it is lower is
helpful for different types

 

and different styles of wines.

 

And some of the
other properties,

 

are just for processing scape.

 

Some of the difficulties is

 

some of the labrusca based
ones have a slip skin.

 

So it's a very difficult pulp,

 

and it will just slip
right out of the skin,

 

which makes pressing extremely
difficult for those varieties.

 

But we're here to talk about
embracing cold climate wine.

 

So things that we do
well, these varieties

 

are almost all very aromatic.

 

Very distinct.

 

Unmistakeable aroma
profiles for these wines,

 

which I think is great
and people need to

 

kind of embrace that and
promote that a little more.

 

And things we can do with
acidic wine with low tannin

 

and low sugar content
is we can make things

 

like sparkling wine.

 

It's a perfect option
for our varieties as is.

 

As well as things like Rosé.

 

I mention here and up and
coming style called Pet Nat,

 

which is a carbonated
form of sparkling wine

 

but not nearly as carbonated.

 

So what they'll do is
they'll take wine as

 

it's nearing fermentation
and bottle it.

 

And the residual sugar
will ferment out,

 

much like a carbonated
bottled conditioned beer.

 

So it's a low
carbonated dry wine,

 

which I hear are quite nice.

 

It's an up and coming trend

 

I see a lot of it coming
out of Europe right now.

 

Dessert wines and ports also

 

work very well
with our varieties.

 

Two of the largest and fastest

 

growing markets
in the wine world

 

are Sauvignon Blanc, which
typically are dry acidic wines,

 

as well as Rosés, which
things like Marquette

 

and Frontenac can
make very nice Rosés.

 

Now we say that
the Midwest is kind

 

of like the wild west
of grape growing.

 

There are no limits.

 

There's nothing telling us
what we can and can't do.

 

So hopefully some creativity and

 

adventurous people will come
out and create some new things.

 

So some statistics on both
Sauvignon Blanc and Rosé.

 

You can see that
the blended table

 

market was up 33% last year.

 

So I think that's a good
opportunity for wineries here

 

to take advantage of that,
as well as to maybe embrace

 

some of the lesser sweet
styles, like Sauvignon

 

Blanc and New Zealand ones,
which tend to be dry and acidic.

 

I should note that while the
blush category is up 33%,

 

White Zinfandel was actually
down 7% over the last year.

 

All that is great,
but one of the things

 

I'm here for is to
talk about quality.

 

So, quality in Wisconsin wine.

 

My purpose is to help improve
the quality of Wisconsin wine,

 

identifying some of the top
quality challenges and issues.

 

So last, when I first got here,

 

I surveyed all the wineries
in the state to get an

 

idea the things they
thought were the top quality

 

challenges for the
state of Wisconsin.

 

These are the issues
that they brought up,

 

and they're the typical
culprits of almost

 

any wine production
area out there.

 

So, oxidation,
sulfide production,

 

volatile acidity,
quality of fruit.

 

A little less pressing
is just wine style,

 

matching the fruit with the type

 

of wine or good style of wine,

 

and just issues of fruit
growing and wine quality

 

So not just figuring out how
to make the grapes survive,

 

but how to grow the
grapes in a way that they

 

can actually
produce better wine.

 

So there's like two layers there
that we're trying to get to,

 

the first layer and
then the second layer.

 

But what is quality mean?

 

And that's one of the challenges
of my position is that

 

quality itself is
kind of a vague term,

 

and it varies a lot
by individual and who
you're talking to.

 

So someone in the
service industry might

 

talk about complexity, body,
weight, food and wine pairing.

 

The Sauvignon Blanc from
New Zealand tastes like

 

it should be a Sauvignon
Blanc from New Zealand,

 

or if the flavor profile is
off, integration and harmony

 

and all these terms that they

 

like to use for
their wine quality.

 

From a producer perspective,
is it free from faults?

 

Is it bottle stable?

 

Did it produce the type of
wine that I was trying to make?

 

That might be what producers are

 

looking for in terms of
what they define quality.

 

Here's two drastically
different types of wine.

 

Screaming Eagle being a thousand
dollar-plus per bottle cult

 

wine out of California
and Beringer producing

 

and $8 bottle of White Zin,

 

When we talk about quality,
which one has more quality?

 

Which is a higher quality wine?

 

Well, it depends on how
you want to look at it.

 

You might say the
Screaming Eagle,

 

because of its complexities and

 

where it's from, is
a high quality wine.

 

But I could easily say that
Beringer is a high quality wine

 

because that wine, I did
tons of analysis on that wine

 

so I can tell you
that a lot of effort

 

and research goes into
that wine production.

 

Very careful about it.

 

A lot of effort goes into it.

 

When you got pick up a bottle
of White Zin from Beringer

 

off the shelf, one bottle
could be produced last month,

 

the other bottle could have
been bottled six months ago,

 

they'll be identical
pretty much.

 

That's a difficult
feat to achieve.

 

That reproducibility that they

 

have should not be understated.

 

So it depends on what you
determine quality to be.

 

When you start talking
about wine faults

 

and things that are bad quality,

 

it gets to be a bit
of fine line between

 

what constitutes complexity
and what's objectionable

 

So somebody might
like a bit of a--

 

character in their
wine; someone else might

 

think it's the worst thing ever.

 

Where that line
is drawn is really

 

dependent upon you
as an individual.

 

Your experience with wine.
How much wine have you drank?

 

How many different regions have

 

you drank it from in
your history with that?

 

As well as perspective.

 

The example that I saw
of perspective is, say,

 

two individuals smell
rosemary in a wine.

 

One person might
associate that with

 

a culinary rosemary chicken or
some other food application,

 

but another person
might compare that with

 

a personal care
product, like a lotion.

 

So which would you rather
have your wine smell like?

 

So, two people smelling the
same thing are going to have two

 

very different ideas on whether
they like that wine or not.

 

And then you get
into physiology,

 

which is every one of us
smells wine differently.

 

What we're sensitive to

 

and not sensitive to
is very individual.

 

So every person's
experience is unique.

 

It makes quality kind of
a challenge to define.

 

So I refer to Dr. Bisson out
of UC Davis who defined GRAY,

 

which is generally
recognized as yucky.

 

So these are the
compounds I focus

 

on when it comes
to Wisconsin wine.

 

As we mentioned before,
here's a few examples or

 

some of the common examples

 

of issues you might
find in wine and cider.

 

So, sulfides, oxidation and
acid aldehyde, cork taint,

 

which is a compound called
trichloroanisole, or TCA

 

if you want to sound hip
for your next dinner party,

 

Brettanomyces, 4-ethylphenol,

 

4-ethylguaiacol, acidic
acid, ethyl acetate, VA,

 

and a few other things
we'll talk about.

 

I don't know if that is very
visible from back there,

 

but sulfides are not a
very pleasant compound.

 

They're usually a result of

 

stressed yeast in
a fermentation.

 

They start kicking out,
start out as rotten

 

eggs all the way down
to canned vegetables.

 

So these are kind of
some of the initial areas

 

of some wine flaws that
you might come across.

 

Definitely probably
not acceptable

 

except in very tiny amounts.

 

Another area that's a
problem is oxygen pressure

 

on the wine during storage as
well as stress fermentations.

 

So if the wine is stored in
an environment that has a lot

 

of oxygen pressure
on it, whether it is
a low grade plastic

 

tank where the oxygen can
transmit through the side

 

or a lot of head space,
ethanol can be converted

 

over to acid aldehyde.

 

Acid aldehyde at low
levels can give kind of

 

a fruitier aspect to your wine.

 

Higher levels start to
smell like rotten apples.

 

Further than that I
get airplane glue,

 

model airplane glue aroma.

 

(Laughter)

 

Nuttiness but generally
considered a flaw,

 

unless you're sherry
where it's expected

 

and needs to be in
very high levels.

 

So wine flaws are
always in kind of

 

an interesting area depending

 

on what you're
trying to achieve.

 

This is a common sort of flaw
that we find in Wisconsin

 

just because of
proper application of
sulfur dioxide as well

 

as minimizing oxygen
exposure, which,

 

as you get on smaller
and smaller scales

 

and more surface area,
tends to be a much bigger

 

challenge for small
wineries to maintain.

 

So TCA is a combination
of mold and chlorine.

 

So a little bit of mold
plus any sort of chlorine

 

in the environment, most people
think it's based from cork,

 

but it could be from
the winery itself.

 

If there's any mold and they use

 

a chlorinated cleaning
component, you can get winery

 

borne TCA as well.

 

Not a very pleasant compound.
It's super potent.

 

It's parts per
trillion sensitivity.

 

Like as low as five
parts per trillion

 

people can start to pick
up on this compound.

 

So like one gram in an
Olympic sized swimming pool

 

is what we can detect.

 

Cork is, you can
see where you might

 

get a little bit of mold
or whatever from the cork.

 

That's an agricultural product.

 

So, some unpleasant
compounds from Brettanomyces.

 

Brettanomyces is
a spoilage yeast.

 

It's present in the environment.
It's on the grapes.

 

If a winemaker is not in a
situation where they have

 

a high pH wine,
Brettanomyces thrives

 

well in higher pH environments.

 

Low pH, they don't
survive as well.

 

Sulfides are good
at maintaining them.

 

But they can produce,
and if there's

 

a lot of residual nitrogen left,

 

one of the things
I didn't mention

 

before was that a
lot of these hybrids

 

actually have a lot
of nitrogen content.

 

You need a certain
amount of nitrogen

 

content to maintain a
healthy fermentation.

 

In a lot of cases, depending
on where it's grown,

 

I've seen fruit out of
Iowa have yeast assimilable

 

nitrogen content, or
primary nitrogen content

 

that the yeast use,
at six to seven times

 

what the yeast actually needs.

 

A lot of that gets leftover
in the final fermentation.

 

If you're not properly
maintaining your wine,

 

it can feed spoilage
organisms pretty well.

 

it can feed spoilage
organisms pretty well.

 

And then you get some
pretty awful things.

 

Unless, of course,
you like sour beer,

 

then those are the things
you're looking for.

 

It depends in what you're into.

 

Another concern here in
Wisconsin and a lot of places

 

is acidic acid
and the conversion

 

of the acidic acid
to ethyl acetate,

 

which smells like
nail polish remover.

 

Acidic acid,

 

if you've got a lot of
oxygen present on your wine

 

and you're not
properly sulfiding it,
then the acetobater

 

can take over and
start kicking it out.

 

Otherwise, stress yeast,
particularly ice wines,

 

can get a bit of VA naturally.

 

It's kind of a natural
process for that.

 

This mousiness is typically
associated with cider.

 

I kind of bring it up because
it's an interesting particular

 

compound because you can't
smell it at the cider pH.

 

So until you taste it,
you won't know it's there.

 

So it's kind of a horrible
shock sort of reality.

 

The pH, and it depends
on the individual,

 

so if you're an individual
who has a higher pH saliva,

 

it raises the pH of
the wine or the cider,

 

and then it becomes
nice and noticeable

 

on the finish of your palate.

 

It's not the greatest way
to finish a sip of wine.

 

Some other common things
are protein formation.

 

Haze formation due to
protein instability

 

due to heating of the wine.

 

Potassium bitartrate are
obviously little crystals

 

you might get if you chill wine.

 

Wineries still put
a lot of effort

 

to remove those from wine.

 

Apparently consumers still might

 

mistake them for
glass particles.

 

It's a lot of effort to do that.

 

A lot of energy goes
into chilling wine down

 

and getting the potassium
bitartrate to settle

 

out of it simply for
cosmetic reasons.

 

You do get a little bit of
acid reduction from that.

 

So wineries who like to do
it kind of get a little bit,

 

take down their
acidity a little bit.

 

But, again, depending
on who you are,

 

sometimes hazes are good.

 

A couple of kind of recent,

 

last five or 10 years, popular

 

wines is a thing
called orange wine.

 

So it's white wine that's
been fermented on its skins.

 

Some of it has been fully
filtered and processed

 

out to look like a
clear wine, but some

 

of it's just left raw,
hazy, and kind of natural.

 

So there's a whole
group of people

 

that get into that.

 

I talked about Pet
Nat a little bit.

 

So you get a wine
that has sediment

 

in it because of
that refermentation

 

of the yeast in the bottle.

 

So the world is kind of
changing in the world

 

of wine of what's
considered acceptable

 

and unacceptable anymore.

 

So you can see
the orange wine on

 

top there is very cloudy.

 

That's intentional.

 

So in terms of
Wisconsin wine and

 

whether or not these GRAY
issues are more common,

 

it's hard to say.

 

I've been to wine
regions all over

 

the country and the world, and

 

I've experienced and encountered
these problems everywhere.

 

The typical issues obviously
are experience in both

 

just recognizing
what these flaws are

 

and how to prevent
them in production.

 

Expertise.

 

One of the things
recognizing that commercial

 

wine production is
a much different

 

animal than home
wine production,

 

which can be a
challenge for some

 

people to make
that transition and

 

realizing there's
a whole new world

 

of technology that they have to

 

learn how to deal
with to get better

 

at producing a commercial wine.

 

A lot of people get into the

 

winemaking world without
having a scientific background.

 

Wine production is a
very scientific thing.

 

So you'll get people who
are close to retirement,

 

decide they want to open a
winery, probably haven't had

 

a chemistry class since
sophomore year of high school.

 

It makes things a little
challenging at times.

 

It's a very capital intensive

 

and expensive proposition
to start a winery.

 

Having proper equipment
and laboratory equipment to do

 

quality wine production
can be a bit of a challenge.

 

So, things we do here.

 

Well, we have
analytical services.

 

So we can do some of the
higher end analysis for you.

 

I do site visits.

 

I go out and I
consult with wineries

 

and help them work
through their challenges.

 

If they wish to contact me and
work on those, we'll do that.

 

Otherwise, I hold workshops
and educational events

 

where we'll address
specific winemaking issues.

 

And then we're working
on some various cold

 

climate specific research

 

to improve the
quality of the wine,

 

to understand how we can make
wine out of these varieties.

 

So, horticulture has
probably presented here,

 

I'm pretty sure at least once
or twice, but some of the things

 

they're working on
is the impact of

 

fruit shading and sun exposure
on the quality of fruit.

 

So they're going
out and pulling the

 

leaves off and leaving some
vines very well shaded,

 

some vines very well exposed,

 

and looking at kind
of the differences of

 

what the chemistry of
those varieties are.

 

They're also looking
at trellising,

 

different types of trellises.

 

Best ways to grow
various varieties.

 

One type of trellis
system might not

 

work well with one
variety than another.

 

They're looking at some pest

 

management as well as disease
management options as well.

 

So on our side over in enology,

 

it was a pretty
easy thing to take

 

the fruit shading
thing and let's

 

make some wine out of it

 

and we can compare
the flavor of that.

 

It's hard to say whether
one is better than the other

 

based on your
personal preferences,

 

but at least we'll be able
to give these examples

 

of shading to winemakers
and they can make decisions

 

about the style of wine
they want to choose

 

based on the results
that we can give them.

 

Looking at the impact
of skin contact

 

and fermentation temperature.

 

And then I will hopefully
focus on the future of wine

 

and research that I was doing
at the University of Minnesota,

 

which was biological acid
management using yeast

 

and bacteria to help
manage acidity in wine

 

and dealing with that high
malic acid content we have here.

 

I guess one of my ultimate
goals is to identify

 

the flavor compounds
in cold climate grapes

 

and understand how viticulture
and enology influences those.

 

So we can give winemakers
a range of options

 

on how to make
their wine based on

 

how flavor is influenced.

 

So some of the research
we're doing right now with

 

an undergraduate group
doing their senior project

 

is actually looking at skin
temperature and skin contact

 

and fermentation temperature
during red wine production.

 

Traditional red wine
production, seven to 14 days

 

of skin contact before
they press it out.

 

Warmer temperatures, 75
to 85 degrees Fahrenheit.

 

And the goal there is to
extract as much tannins

 

and phenols and color and
flavors from the grapes.

 

One of the things I
mentioned earlier is

 

that we don't have
a lot of tannins

 

and we have a lot of color.

 

It doesn't make
sense for us to be

 

using standard vinifera
practices to be making wine.

 

So these undergraduates,
a very ambitious group of

 

undergraduates that we
have in the department,

 

were evaluating the
color, phenolic content,

 

and flavor differences
in two varieties of wine,

 

Marquette and Frontenac, and
looking at cool fermented

 

and ambient temperature
fermentations

 

at different skin contact times.

 

They're still in
process with that.

 

Hopefully we'll have those
results and we can bring it to

 

Winemakers for their
annual meeting this winter.

 

It's going to be impossible
to read, but there's an arrow

 

pointing at the skin and then
there's a long list of things

 

that we find in the skin.

 

That's where the
skin contact research

 

comes into play here is,
are all those compounds

 

in the skins of hybrid varieties

 

things we actually
want in the wine?

 

And is it better to do
a shorter skin contact

 

versus a longer skin
contact fermentation?

 

We know that from other
research that exposing

 

the fruit to more sun
does lower acidity,

 

gives it more
sugar, and increases

 

the phenolic content

 

and gives it a different
flavor profile.

 

The three varieties
that we're looking at

 

for this year are Marquette,
Frontenac, and Petite Pearl.

 

Horticulture is examining
the basic chemistry

 

of these things throughout
the growing season.

 

And we're going to take them,
we're fermenting them right now,

 

and we're going to look at

 

the flavor differences
and see if there

 

is a change in the kind
of hybrid herbaceousness

 

and vegetable character of
some of these varieties,

 

and whether or not
shading or unshading

 

is actually a positive
or negative attribute.

 

So in terms of tannins,
Cornell University

 

is doing a lot of
research on tannins.

 

I say there's not a lot of
tannins in the final wine,

 

but there's actually a
fair amount of tannins

 

in the grapes themselves.

 

An active area of research
is trying to figure out

 

why there's not tannin
in the final wine.

 

There are hypotheses, and
research indicates that

 

there's a grape solid, a
grape particle that during

 

fermentation is binding
with the tannins

 

and is causing those
tannins to settle

 

out of the fermentation.

 

They've done research
where they've added

 

tannins at the start
of the fermentation

 

and measured how much
tannins were afterward,

 

and it takes a tremendous

 

amount of tannins
to get any result.

 

So these particles are very
powerful and very active.

 

So research that I've done
quite a bit of back in Minnesota

 

and will continue to do
so moving forward here

 

at the University of
Wisconsin is looking

 

at acid management
because one of the things

 

that we're having one of
the biggest challenges

 

we have are the high
acidity of these grapes

 

and grape varieties,
particularly malic acid.

 

It's not just so
much purely sourness

 

that we got to worry about,

 

but it's the interaction of
acid with other compounds.

 

So the more acid we
have in there, the more

 

likely you're
going to taste some

 

of the bitterness
compounds that might

 

be present and the more
likely you're going to

 

have some astringency
because malic acid

 

itself has an
astringent character.

 

So in terms of what we have

 

available to us
to manage acidity,

 

there's really, once
it gets to the winery,

 

there's a couple, a few
methods we can deal, use.

 

So there's some
chemical methods,

 

and then there's using biology.

 

So as far as chemical
deacidification,

 

we have potassium bicarbonate,
calcium carbonate or chalk.

 

Otherwise, you can use water

 

and try to reduce
acidity that way,

 

or add a lower acid.

 

Grape to it.

 

In terms of biological
deacidification,

 

we have the bacteria in yeast.

 

So potassium bicarbonate,

 

calcium carbonate are
two of the main ones.

 

The important factor
to realize there

 

is that most of those only
work on tartaric acid.

 

They won't reduce the
malic acid content.

 

So if you have a variety
of lots of malic acid,

 

we can't really reduce
that acid very far.

 

And it leaves a
much higher level

 

of malic acid ratio
there, which kind of gives

 

it a more harshness to the wine.

 

So we're trying to
use biological methods
to reduce acidity.

 

We're looking at yeast
and bacteria that convert

 

malic acid to some
other byproduct.

 

So, bacteria convert
malic acid to lactic acid,

 

and then there's yeast that
convert malic acid to ethanol.

 

And we can do that, and
there are yeast strains

 

that have been identified
that do that very well.

 

There's some nontraditional
wine strains.

 

So Saccharomyces is the
traditional strain we use

 

for wine production
and beer production.

 

But there's a strain
of yeast called

 

Schizosaccharomyces pombe,

 

which does a great
amount of malic

 

to ethanol fermentation.

 

And then research here
potentially in the genetics

 

department might identify
some yeast strains and

 

produce some yeast
strains that can do

 

some biological acid
management as well.

 

So there are certain strains
out there that have been

 

identified that do some
level of acid reduction.

 

71B I've used in the
past can reduce acidity

 

by two grams per liter,

 

which is a significant
amount of acid reduction.

 

Then there's
malolactic fermentation

 

using malolactic bacteria.

 

And, besides acid
reduction, winemakers use

 

malolactic bacteria for flavor

 

and style as well as
microbial stability.

 

That's just one less thing
that an organism can utilize

 

from fermentation once
it's gotten bottled.

 

And there's a range
of organisms out

 

there capable of
malolactic fermentation.

 

Lots of lactic acid bacteria.

 

A strain that we commercially
use is Oenococcus.

 

So malolactic
fermentation is not

 

really actually a fermentation.

 

It's kind of a misnomer.

 

Most fermentations take
sugar and convert it

 

to an acid so it
actually increases

 

the acidity of what
you're trying to do.

 

So malolactic fermentation is
actually reducing the acidity,

 

and it does that using an enzyme

 

that converts to malic
acid or lactic acid.

 

Some other byproducts or
other compounds there.

 

It can also begin to chew
on and use to produce

 

other compounds.

 

It can break citric
acid down into diacetyl

 

and pyruvate and acidic acid.

 

I bring up diacetyl
because it's a compound

 

that's produced and it
masks fruity aroma in wine.

 

So we look at using bacteria
to manage acidity in wine.

 

This is a byproduct
that's a negative aspect.

 

So how do we manage
your diacetyl production

 

and malolactic fermentation
so that it doesn't decrease

 

the fruitiness of wine?

 

Especially if
you're going to use

 

it on Rosé or a
white wine where you

 

want to enhance fruitiness
as much as possible.

 

A couple of formulas.

 

Always feels to good to
throw some of those on there.

 

But, basically, we're converting
malic acid, lactic acid

 

just by using the
enzyme to cleave

 

off one of the carbon
groups on the malic acid.

 

And so the research
that I look into

 

then is the timing of
malolactic bacteria additions.

 

One of the reasons why
we look into that is that

 

we know that yeast
can convert diacetyl

 

to another compound that's
not nearly as strong smelling.

 

It doesn't have as nice a name
as diacetyl, 2, 3-butanediol.

 

It's helpful to know that if

 

the yeast is present
we can alter the timing

 

of malolactic fermentation
such that we can remove

 

that diacetyl and be able
to apply it to fruity wines

 

that we don't want the
diacetyl mass character.

 

The challenge, though,
is that a lot of people

 

around here make sweet wines,

 

and then to prevent
further fermentation

 

they'll use the compound
potassium sorbate.

 

It inhibits further yeast
activity in the wine.

 

The only problem is is that
they're using that typically

 

because they don't feel
confident enough in their

 

sterile filtration to
prevent further fermentation

 

once the wine is bottled.

 

Consequently, if there is
a bacterial infection or a

 

bacterial present with
wines that have potassium

 

sorbate, then there's
a geranium taint

 

issue that can result.

 

So they convert that
sorbic acid into a

 

compound that smells a
lot like geranium leaves.

 

When I was at the
University of Minnesota,

 

my graduate research project was
looking at malolactic bacteria

 

additions and timing on
Marquette wine production.

 

And one of the things I
found was that it did reduce

 

the time of malolactic
fermentation completion.

 

That's another advantage of
a co-inoculation strategy,

 

which is adding bacteria
within 24 hours after adding

 

your yeast versus the
traditional method,

 

which is to add
bacteria after alcoholic

 

fermentation is completed.

 

The impact, no impact on
the liking of the wine

 

and it reduced malolactic
fermentation overall time.

 

So, knowing that there's
all these advantages

 

to using bacteria, looking
at how we can apply that

 

to white and sweet
wine production

 

would be great for the
Wisconsin wine industry.

 

The other biological method

 

that's out there is
Schizosaccharomyces pombe,

 

which is a commercial
product under ProMalic.

 

It's naturally considered
a spoilage organism.

 

So what this company did was

 

encapsulate it in
an alginate shell.

 

So we can put them
into these mesh bags,

 

add them to the wine.

 

It starts doing the malo
to ethanol fermentation,

 

and then once we're complete or

 

reached the desired
level of acid reduction,

 

we can pull it out and
hopefully take all that yeast

 

with us so that it
doesn't result in some of

 

the negative sensory
aspects that people

 

believe it can produce.

 

I would like to research that

 

a bit further and actually look

 

at other strains and see if
there's a way to actually use

 

it in its natural form without

 

the alginate shell to see if we

 

can produce wines that
have low acid content.

 

I've used the ProMalic
in the past and have had
some success with it.

 

So I've taken La Crescent
that had a TA, titratable

 

acidity probably in
the 12 to 14 range,

 

and had a final product
about 6.5 grams per liter.

 

So it does work, and
it can work quite well.

 

But just a matter of
what are the fermentation

 

conditions that we can
succeed with that as well as

 

minimizing flavors
and trying to tailor

 

that to specific wine styles.

 

At one point there was a
group of researchers out of

 

British Columbia who actually
took both the aspects of

 

Oenococcus and S. pombe
and created a genetically

 

modified yeast called MLO1.

 

It was on the
market for a while.

 

It got approved by the
Tobacco, Tax, and Trade Bureau.

 

I believe it's still
potentially out there.

 

This was originally sold
under the Red Star brand,

 

which eventually
got bought out by

 

a French company called Linares

 

French aren't very happy
about genetically modified

 

creatures so this kind
of disappeared from

 

the market after that merger.

 

But it is something
that was out there

 

and was approved by the TTB.

 

One of the things that
we hopefully can develop

 

here is that the
Hittinger Lab upstairs

 

has found ways to
create some novel

 

yeast strains under
a new technology.

 

So right now it's being applied
mainly to beer production

 

but could some of these
yeasts actually enhance

 

and produce interesting
wines and ciders.

 

So hopefully we'll be
able to start researching

 

that in the future as well.

 

So, embracing the cold
climate wine production,

 

looking at and identifying
processing techniques

 

that influence flavor,
managing acidity,

 

coming up with some
new yeast strains.

 

And I like to say there's a
lot of room for experimentation

 

and creativity in this industry.

 

Canada, for example,
some researchers

 

up there are looking
at grape drying.

 

The Amarone raisin
style wine production.

 

Instead of just taking
grapes and putting them

 

into the attic of a barn and
blowing air through them,

 

they've looked at a wide
range of doing that,

 

including using retired tobacco

 

dryers to rapidly dry
down grapes and create

 

different types of
wines and take advantage

 

of the properties that those
grapes have available to them.

 

So there's
opportunities out there,

 

and there's not restrictions.

 

Not Europe where
you have to grow

 

your grapes a certain way,

 

produce your wines
a certain way.

 

There's all sorts of
options and opportunities.

 

So, if you're, I guess,
an adventurous sort,

 

growing grapes and wine
and making wine here in

 

Wisconsin is kind of
a great place to be.

 

And with that, I thank
you all for coming.

 

There's my contact information,

 

if you have any other questions
about making wine in Wisconsin.

 

(Applause)