WEBVTT 00:04.137 --> 00:05.772 >>NARRATOR:In the spring of 2008, 00:05.772 --> 00:08.909 an 84-foot pleasure boatdeparted from Fort Lauderdale 00:08.909 --> 00:11.445 bound for the Caribbean. 00:11.445 --> 00:12.980 30 miles south of Miami, 00:12.980 --> 00:15.816 it strayed from markednavigation channels 00:15.816 --> 00:20.454 into the shallow watersof Biscayne National Park. 00:20.454 --> 00:24.925 Suddenly, running at full speed,it collided with a coral reef 00:24.925 --> 00:27.127 near Elliot Key. 00:27.127 --> 00:30.998 Corals, sponges and sea fanswere instantly obliterated 00:30.998 --> 00:35.235 as the boat's twin propellersplowed through the reef. 00:35.235 --> 00:39.206 The engines were disabled,and the powerless vessel 00:39.206 --> 00:44.111 drifted in the wind untilgrounding on a second reef. 00:44.111 --> 00:48.515 Here, the wind and wavesrocked the boat on its hull, 00:48.515 --> 00:51.552 shattering the ancientcoral mounds and pounding 00:51.552 --> 00:55.022 the reef into rubble. 00:55.022 --> 01:00.394 A coral reef that had takencenturies to grow 01:00.394 --> 01:03.397 was destroyed in just moments. 01:07.401 --> 01:09.002 Two-and-a-half years later, 01:09.002 --> 01:13.240 coral researchers and resourcemanagers are searching 01:13.240 --> 01:18.478 for solutions to help theocean's declining coral reefs. 01:18.478 --> 01:20.514 Can new technologies 01:20.514 --> 01:23.617 and naturally occurringbiological mechanisms 01:23.617 --> 01:27.421 help restore lost coralcommunities? 01:27.421 --> 01:29.823 And can ecological balance 01:29.823 --> 01:32.926 be returned to Florida'scoral reefs? 01:57.884 --> 02:00.787 >>Major funding for thisprogram was provided 02:00.787 --> 02:02.889 by the Batchelor Foundation, 02:02.889 --> 02:05.592 encouraging peopleto preserve and protect 02:05.592 --> 02:09.563 America's underwater resources. 02:17.571 --> 02:20.741 >>NARRATOR: Biscayne NationalPark is an undersea garden 02:20.741 --> 02:27.080 filled with multicoloredsponges, corals and sea fans. 02:27.080 --> 02:29.983 At the northern extentof the Florida Keys reef tract, 02:29.983 --> 02:34.054 its sea grass beds, coral reefsand mangrove shorelines 02:34.054 --> 02:37.991 cover more than 170,000 acres, 02:37.991 --> 02:41.128 a gemin the national park system, 02:41.128 --> 02:45.132 but an area with a historyof damaging boat groundings. 02:51.905 --> 02:56.476 Corals in Biscayne National Parkand on a global scale 02:56.476 --> 03:00.781 are in a serious stateof decline due to stresses 03:00.781 --> 03:05.519 like climate change, diseaseoutbreaks and overfishing. 03:05.519 --> 03:10.857 Since the late 1970s,close to 98% of staghorn 03:10.857 --> 03:14.795 and elkhorn coralshave disappeared from the reefs 03:14.795 --> 03:17.330 in Florida and the Caribbean. 03:17.330 --> 03:20.834 Because corals are having sucha difficult time persisting, 03:20.834 --> 03:25.172 resource managers wantto restore boating impacts 03:25.172 --> 03:30.177 like the 2008 grounding sitein the park. 03:32.212 --> 03:34.881 >>When the vessel grounded,it crushed up the surface 03:34.881 --> 03:39.953 of the reef into a rubble field,and we're trying to use 03:39.953 --> 03:45.959 a combination of adhesivesto stabilize that rubble. 03:47.294 --> 03:49.696 We're collecting the rubbleinto mounds 03:49.696 --> 03:53.700 like this and like this... 03:55.469 --> 04:00.607 and we'll be using a combinationof cement and natural sponges 04:00.607 --> 04:03.977 that are found right hereon the reef 04:03.977 --> 04:08.982 to bind this rubble to stabilizeit through time. 04:12.119 --> 04:15.021 Right now, the rubble moundsare just in the process 04:15.021 --> 04:19.126 of being put together. 04:19.126 --> 04:22.162 We're installing rebar stakeslike this that will help us 04:22.162 --> 04:25.899 monitor them through time. 04:25.899 --> 04:29.136 And we'll be looking at suchthings as coral recruitment, 04:29.136 --> 04:33.306 the stability of the moundsthemselves, if they change 04:33.306 --> 04:35.308 in size and shape and height. 04:37.878 --> 04:39.713 And we'll probably monitor these 04:39.713 --> 04:42.716 for a period of at leastfive years. 04:51.224 --> 04:53.693 >>NARRATOR: Researchersat the University of Miami 04:53.693 --> 04:57.230 have developed one of the firstunderwater applications 04:57.230 --> 05:00.433 for mosaic imaging,a technique comparable 05:00.433 --> 05:03.737 to aerial photographyon the land. 05:03.737 --> 05:07.541 This technology will createuseful perspectives 05:07.541 --> 05:14.047 of the coral reef and groundingsite in Biscayne National Park. 05:14.047 --> 05:17.784 The diver swims back and forthin a lawnmower-like pattern, 05:17.784 --> 05:22.789 recording overlapping imagesof the underwater landscape. 05:22.789 --> 05:24.991 Later, at the universitylaboratory, 05:24.991 --> 05:29.262 a single seamless imageof the reef is produced. 05:29.262 --> 05:31.932 >>This is basicallyan unprecedented baseline, 05:31.932 --> 05:34.467 in terms of the amountof information that you can get 05:34.467 --> 05:37.504 in a very short amount of time. 05:37.504 --> 05:40.841 >>NARRATOR: Three-dimensionalimages can be created over time 05:40.841 --> 05:44.344 to show reef areaswhere corals grow best, 05:44.344 --> 05:50.016 the places where restorationis most likely to succeed. 05:50.016 --> 05:54.554 New growths of soft coralsand sponges are returning 05:54.554 --> 05:58.725 to the grounding site, butthe boat's most damaging impacts 05:58.725 --> 06:01.928 are still evident. 06:01.928 --> 06:04.898 >>The damage extends severalhundreds of square meters, 06:04.898 --> 06:08.635 and really, there's no easy wayto document that. 06:08.635 --> 06:11.304 Here, we're able to usethe video and the stills 06:11.304 --> 06:15.141 to really cover the entire area,both the affected area, 06:15.141 --> 06:17.811 and also the communityaround it, so that we know 06:17.811 --> 06:23.383 what this area should look likewhen it's fully restored. 06:23.383 --> 06:25.619 >>NARRATOR: Coral reefrestoration gained traction 06:25.619 --> 06:28.588 in the Florida KeysNational Marine Sanctuary 06:28.588 --> 06:31.725 with marine biologistslike Harold Hudson, 06:31.725 --> 06:34.728 "The Reef Doctor,"and Ken Nedimyer. 06:38.131 --> 06:42.135 Hudson, a restoration specialistfor the sanctuary, 06:42.135 --> 06:45.005 perfected a designfor "reef modules" 06:45.005 --> 06:47.474 that were usedfor the structural repair 06:47.474 --> 06:50.810 of a large vesselgrounding site, the Wellwood, 06:50.810 --> 06:55.515 on Molasses Reef near Key Largo. 06:55.515 --> 06:59.552 Nedimyer performedthe biological restoration 06:59.552 --> 07:04.824 with transplanted coralsgrown in his undersea nursery. 07:04.824 --> 07:07.193 His restoration planwas validated 07:07.193 --> 07:10.163 when it was discovered thatthe transplants on the reef 07:10.163 --> 07:16.036 were spawning several yearssooner than expected. 07:16.036 --> 07:17.037 >>I was real excited. 07:17.037 --> 07:18.405 I thought, man, this is amazing, 07:18.405 --> 07:21.308 because this is exactly whatwe've been trying to say 07:21.308 --> 07:22.709 that we're going to do, 07:22.709 --> 07:27.881 which is reestablish thesespawning populations of corals. 07:27.881 --> 07:31.351 It brought a whole new lifeto the possibility 07:31.351 --> 07:33.820 of not just putting coralsback on the reef, 07:33.820 --> 07:36.856 but reestablishing breedingpopulations that could then 07:36.856 --> 07:41.594 repopulate otherdownstream areas. 07:44.597 --> 07:47.267 >>NARRATOR: Nedimyer's coralnursery is the largest 07:47.267 --> 07:52.138 in the country, and a modelfor many coral aquaculturists. 07:52.138 --> 07:55.842 Here, you encounter 100 rowsof coral fragments 07:55.842 --> 07:58.745 mounted on concrete blocks. 07:58.745 --> 08:00.547 Each coral is attachedto a disc, 08:00.547 --> 08:04.584 with a label designatingits origin and genotype, 08:04.584 --> 08:06.152 or genetic properties. 08:10.423 --> 08:12.993 More than a thousand staghorncuttings are strung 08:12.993 --> 08:16.796 from six line nurseriessuspended in the water column 08:16.796 --> 08:22.402 by floats, and cross-tiedwith horizontal lines. 08:22.402 --> 08:24.304 >>On a line nursery,they grow down, 08:24.304 --> 08:26.806 they grow to the side, they growall different directions. 08:26.806 --> 08:29.542 And another thing that's niceabout them-- if a turtle 08:29.542 --> 08:33.980 or a shark or a fish bumpsagainst them on the line, 08:33.980 --> 08:35.882 they just bounce out of the wayand they swing right back 08:35.882 --> 08:37.350 to where they were. 08:37.350 --> 08:40.620 If a turtle bumps againsta coral that's firmly mounted 08:40.620 --> 08:44.457 on a disc, it'll break it off. 08:45.959 --> 08:48.728 >>NARRATOR: Nedimyer cultivatesstaghorn coral, 08:48.728 --> 08:51.131 one of the principalreef building corals 08:51.131 --> 08:53.433 and one listedas threatened 08:53.433 --> 08:56.369 under the EndangeredSpecies Act. 08:56.369 --> 08:58.738 He cultivates themin his nursery for a year, 08:58.738 --> 09:02.442 then transplants themto the reef. 09:04.811 --> 09:07.814 >>One of the things we needto be doing is preserving 09:07.814 --> 09:10.550 the genetic diversitythat we still have. 09:10.550 --> 09:14.020 And if we don't preserve as muchof that as possible right now, 09:14.020 --> 09:18.024 we won't have anything to workwith in ten or 15 years. 09:24.097 --> 09:27.167 >>NARRATOR: Research AssociateTom Capo and colleagues 09:27.167 --> 09:30.737 at the University of Miami'sexperimental hatchery 09:30.737 --> 09:34.741 are preserving the geneticdiversity of orphaned corals 09:34.741 --> 09:38.411 whose survival was threatenedby storms, boat groundings 09:38.411 --> 09:40.647 and anchor pulls. 09:40.647 --> 09:42.615 Some were recoveredfrom the grounding site 09:42.615 --> 09:44.851 in Biscayne National Park. 09:44.851 --> 09:47.320 Researchers are tryingto answer questions 09:47.320 --> 09:53.059 about a destructive phenomenonknown as coral bleaching. 09:53.059 --> 09:54.060 >>What's going on? 09:54.060 --> 09:55.161 Is it disease? 09:55.161 --> 09:57.464 Is it some sort of water qualityparameter? 09:57.464 --> 10:00.733 And many of these questionscannot be answered easily 10:00.733 --> 10:01.968 in the field. 10:01.968 --> 10:06.773 So the goal is to havea coral genetic bank, 10:06.773 --> 10:09.476 a coral resource that we can 10:09.476 --> 10:12.312 provide genetically maintainedstrains of coral 10:12.312 --> 10:15.048 to bona fide researchers aroundthe world, so that they 10:15.048 --> 10:17.450 can look at these problems,at these issues, 10:17.450 --> 10:20.587 and look at themin a scientific way. 10:20.587 --> 10:24.424 >>NARRATOR: In a partnershipbetween Biscayne National Park 10:24.424 --> 10:26.826 and the University of Miami, 10:26.826 --> 10:30.797 trained volunteers assist Capowith a labor-intensive process 10:30.797 --> 10:35.201 known as "fragmentation"to produce a large number 10:35.201 --> 10:38.104 of small coral specimens. 10:38.104 --> 10:40.807 >>You should have one readyfor him now. 10:40.807 --> 10:43.910 I mean, he should not stop. 10:43.910 --> 10:46.112 >>NARRATOR:Travertine plates are marked, 10:46.112 --> 10:49.983 drilled and fitted with passiveintegrated transponders, 10:49.983 --> 10:54.988 or PIT tags, that will giveeach coral a unique identity. 10:58.024 --> 11:00.293 The large corals are cutinto fragments, 11:00.293 --> 11:04.230 each about an inch-and-a-halfin diameter. 11:04.230 --> 11:09.302 The bottom surface is shapedon a tile saw. 11:09.302 --> 11:12.338 The fragmentis thoroughly dried... 11:13.907 --> 11:16.910 super-glued to a PIT tag plate, 11:16.910 --> 11:20.780 and catalogued for futurereference. 11:22.815 --> 11:25.919 Half of the fragmentswill be transplanted 11:25.919 --> 11:30.089 at the coral restoration sitein Biscayne National Park. 11:30.089 --> 11:33.660 The other half will remainat the facility for monitoring 11:33.660 --> 11:38.064 and lab studies to help Capoand coral researchers 11:38.064 --> 11:41.901 answer anotherdifficult question. 11:41.901 --> 11:43.836 >>How can we enhanceall this to make 11:43.836 --> 11:47.407 the only real reef tractin the continental United States 11:47.407 --> 11:50.543 a viable place ratherthan seeing it deteriorate 11:50.543 --> 11:55.782 like it's been doingover the past ten years? 11:55.782 --> 11:59.152 >>NARRATOR: One of Capo'sresearch partners, Martin Moe, 11:59.152 --> 12:01.955 a marine biologistin the Florida Keys, 12:01.955 --> 12:05.458 believes he has an answer. 12:05.458 --> 12:10.630 >>In 1983, there wasthis tremendous plague 12:10.630 --> 12:12.765 and it hit only one organism. 12:12.765 --> 12:17.370 And that was the long-spinedsea urchin, Diadema antellerum, 12:17.370 --> 12:20.073 that occupied all the reefsof the Caribbean, 12:20.073 --> 12:22.275 the Bahamas, Florida. 12:22.275 --> 12:26.446 They were all very denselyoccupied by Diadema. 12:26.446 --> 12:30.450 And Diadema performedthe essential task 12:30.450 --> 12:33.052 of cleaning the algaeoff the reefs 12:33.052 --> 12:37.257 and conditioning the substrateso that it would accept 12:37.257 --> 12:40.260 and encourage coral growth,and the growth 12:40.260 --> 12:43.363 of many different kinds of fishand invertebrates. 12:43.363 --> 12:48.368 Within one year, 98% of allthe Diadema antellerum 12:48.368 --> 12:50.570 from the mouthof the Panama Canal 12:50.570 --> 12:54.173 all the way up to Bermudawere history, were toast. 12:54.173 --> 12:55.308 They all died quickly. 12:57.510 --> 13:00.246 >>NARRATOR: At first,divers like Ken Nedimyer 13:00.246 --> 13:04.917 were happy about the declineof the sharp-spined creatures. 13:04.917 --> 13:07.020 >>As much as I don't likegetting stabbed by them, 13:07.020 --> 13:09.355 I think they're importantand I want to have them back. 13:09.355 --> 13:12.558 >>NARRATOR: Like sheep grazingin a pasture, 13:12.558 --> 13:16.296 Diadema sea urchins grazethe macroalgae that grow 13:16.296 --> 13:19.899 on the reefs and inhibitcoral reproduction. 13:19.899 --> 13:21.634 >>A lot of the problemsare directly related 13:21.634 --> 13:23.369 to the lack of sea urchins. 13:23.369 --> 13:25.905 And if you fix all the waterquality issues, 13:25.905 --> 13:27.140 and fix all of theseother things, 13:27.140 --> 13:29.242 but you don't fixthe sea urchin problem, 13:29.242 --> 13:31.010 you're not goingto have a coral reef. 13:31.010 --> 13:34.714 Sea urchins playa really important role. 13:34.714 --> 13:38.318 >>Ken and I came to the pointof view that the loss 13:38.318 --> 13:43.890 of the Diadema was criticalto the decline of the reefs. 13:43.890 --> 13:48.227 Now, our coral reef declinecomes from many factors, 13:48.227 --> 13:50.496 not just the loss of Diadema. 13:50.496 --> 13:53.566 But that is certainly oneof the most critical elements, 13:53.566 --> 13:56.769 because it's the Diademathat maintain that balance 13:56.769 --> 14:01.007 between coral and algae growth. 14:01.007 --> 14:02.508 >>NARRATOR:With support from 14:02.508 --> 14:05.511 the Florida Keys NationalMarine Sanctuary, 14:05.511 --> 14:09.582 Nedimyer and Moe didtwo experimental reef studies 14:09.582 --> 14:12.785 where they reintroducedthe Diadema. 14:12.785 --> 14:17.123 A year later,the findings were remarkable. 14:17.123 --> 14:21.527 Juvenile corals increasedby more than 150%; 14:21.527 --> 14:24.530 coralline algae, whichencourages coral settlement 14:24.530 --> 14:26.699 and growth, was sharply higher; 14:26.699 --> 14:33.139 and macroalgae was reducedfrom 11% to less than 2%. 14:33.139 --> 14:35.041 >>I started withthe Florida Board 14:35.041 --> 14:42.348 of Conservation MarineLaboratory back in 1962. 14:42.348 --> 14:45.151 Florida was a very differentplace back then. 14:45.151 --> 14:48.755 I worked with the Keys,and the Keys were magical. 14:48.755 --> 14:53.659 I remember going out and divingaround John Pennekamp area, 14:53.659 --> 14:58.831 and there were huge standsof elkhorn coral, palmata. 14:58.831 --> 14:59.932 Beautiful. 14:59.932 --> 15:02.335 And it was sucha tremendous environment. 15:02.335 --> 15:06.839 And when I came backin the late '90s, 15:06.839 --> 15:08.875 the reef was so much different. 15:08.875 --> 15:13.813 And it was so sad to see whatwas a glorious coral environment 15:13.813 --> 15:19.552 just become, for the most part,a lot of algae covered rocks. 15:19.552 --> 15:23.389 It seemed like the best thingthat I could do was to work 15:23.389 --> 15:25.892 with the Diadema, because nobodyelse was doing it 15:25.892 --> 15:30.129 and it was somethingessential to do. 15:30.129 --> 15:32.165 >>NARRATOR: Martin Moeis committed to perfecting 15:32.165 --> 15:35.134 the technology to reproduce,or culture, 15:35.134 --> 15:38.137 Diadema in the laboratory. 15:38.137 --> 15:41.974 Today, he beginsa new culture process, or run, 15:41.974 --> 15:45.978 by selecting Diademathat are ready to spawn. 15:45.978 --> 15:50.983 Immersing them in a tub ofwarm water stimulates the spawn. 15:50.983 --> 15:52.685 >>Oh, yeah. 15:52.685 --> 15:53.753 Now that's a male. 15:53.753 --> 15:56.756 You can see the white spermthere. 16:04.030 --> 16:07.900 Yes, sir, that's a female. 16:07.900 --> 16:10.102 We've got spawning--first three. 16:10.102 --> 16:12.572 How about that? 16:12.572 --> 16:16.142 Okay, this is the productfrom the spawn. 16:16.142 --> 16:18.678 Apparently, a male and a femaleboth went, 16:18.678 --> 16:23.616 which means thatI should have eggs in here. 16:23.616 --> 16:27.119 Oh, yes, that is nice. 16:27.119 --> 16:29.956 I can see fertilizationmembranes are present 16:29.956 --> 16:34.594 around every egg, so we knowwe have had a good spawn. 16:34.594 --> 16:36.963 We know we've got good eggs. 16:36.963 --> 16:39.098 Out of, let's say,five million eggs 16:39.098 --> 16:42.101 that were spawned here,which is a pretty good estimate, 16:42.101 --> 16:45.471 and out of those five million,you'd probably be lucky 16:45.471 --> 16:49.442 if one or two made it,in nature, all the way 16:49.442 --> 16:52.612 through the processand became a juvenile. 16:52.612 --> 16:56.716 And then, you'd probably belucky if one of those survived 16:56.716 --> 17:00.720 into adulthood--very lucky, actually. 17:06.926 --> 17:07.927 >>NARRATOR:The following morning, 17:07.927 --> 17:11.831 he counts the developingembryos. 17:11.831 --> 17:15.134 >>The eggs hatch in about18 to 24 hours. 17:15.134 --> 17:18.170 They become blastulas;they become prisms, 17:18.170 --> 17:20.673 which is the initial urchinshape. 17:20.673 --> 17:22.542 >>NARRATOR:The embryos are transferred 17:22.542 --> 17:26.445 to three larvae culture tankswhere they will eat and grow 17:26.445 --> 17:29.682 for the next 35 to 45 days. 17:29.682 --> 17:32.385 But unlike other typesof invertebrates, 17:32.385 --> 17:35.821 Diadema larvae will dieif they settle on the bottom 17:35.821 --> 17:37.156 of the tanks. 17:37.156 --> 17:41.327 Finding a method to mimicthe natural ocean current 17:41.327 --> 17:45.164 to keep the larvae in suspensionhad been Martin's biggest trial 17:45.164 --> 17:47.300 during his early cultureattempts. 17:47.300 --> 17:49.969 Maintaininga sterile environment 17:49.969 --> 17:51.737 had been another problem. 17:51.737 --> 17:55.908 Entire runs had failedin the culture tanks. 17:55.908 --> 17:59.445 The weeks ahead will againtest his system 17:59.445 --> 18:03.449 and the Diadema's abilityto survive. 18:07.720 --> 18:11.757 Meanwhile, the fragmented coralsfrom the University of Miami 18:11.757 --> 18:16.562 have arrived at the groundingsite in Biscayne National Park. 18:23.202 --> 18:26.205 Volunteer helpers swimwith buckets of cement 18:26.205 --> 18:31.711 and crates of tilesto the transplant areas. 18:31.711 --> 18:35.314 Another group has reattachedbroken pieces 18:35.314 --> 18:39.185 and transplanted nursery-raisedcoral to the site. 18:39.185 --> 18:42.722 And resource managersare betting on the feasibility 18:42.722 --> 18:45.958 of a new restoration technique. 18:47.793 --> 18:50.630 >>We're going to be usingsponges to bind the rubble 18:50.630 --> 18:52.832 that was createdby this grounding incident. 18:52.832 --> 18:54.333 And this is the first timewe've tried this, 18:54.333 --> 18:57.069 and to our knowledge,the first time that sponges 18:57.069 --> 18:59.472 have been used in anactual restoration project 18:59.472 --> 19:00.940 for this purpose. 19:00.940 --> 19:04.677 >>NARRATOR: Sponges are knownto play an ecological role 19:04.677 --> 19:08.614 in binding rubblein a natural reef setting. 19:08.614 --> 19:11.317 There's also evidence that coralwill settle and grow 19:11.317 --> 19:16.522 more favorably on an areathat's been bound by sponges. 19:21.293 --> 19:24.397 >>Right here, we've markeda sponge where we cut 19:24.397 --> 19:27.500 a fragment off yesterday. 19:27.500 --> 19:29.635 We've tagged and markedthis parent colony, 19:29.635 --> 19:32.605 so that we can track itthrough time and see 19:32.605 --> 19:37.977 how that lesion heals, justto make sure it's doing okay. 19:37.977 --> 19:41.013 The sponge fragment we tookfrom there we've attached 19:41.013 --> 19:43.015 over in our sponge nursery. 19:46.352 --> 19:51.924 Each tile has nine PVC trees. 19:51.924 --> 19:56.896 We attach one sponge fragmentto each PVC tree. 19:59.398 --> 20:03.102 We cut these sponge fragmentsoff of the parent colonies 20:03.102 --> 20:05.104 that I showed you earlier. 20:05.104 --> 20:09.475 We're trying to getthe cut surface in contact 20:09.475 --> 20:15.581 with the paver stone at leastas closely as possible. 20:15.581 --> 20:19.652 Our colleagues have shownthat those cut surfaces bind 20:19.652 --> 20:23.122 to whatever they're in contactwith very quickly, 20:23.122 --> 20:26.258 if all goes well. 20:26.258 --> 20:30.096 So we hope to come back and seethese sponges growing down 20:30.096 --> 20:34.400 onto the tiles, and alsoup starting to branch out. 20:37.336 --> 20:40.272 And the idea is that, in time,once the sponges grow 20:40.272 --> 20:44.243 and start getting larger,that you can then cut fragments 20:44.243 --> 20:51.016 off of these and use themin restoration projects. 20:51.016 --> 20:53.786 We tried to get allof our fragments cut 20:53.786 --> 20:57.156 to approximately ten centimetersin size. 20:57.156 --> 21:02.261 We harvested these fragmentsyesterday, and some of them 21:02.261 --> 21:07.666 are starting to showa slightly blackish color. 21:07.666 --> 21:11.637 We're not sure if that'sa good thing or a bad thing. 21:11.637 --> 21:15.341 We're going to be watchingthese sponges carefully-- 21:15.341 --> 21:17.810 on a daily basisin the beginning-- 21:17.810 --> 21:22.414 and then probablyevery week or so 21:22.414 --> 21:26.652 to see how they surviveand grow. 21:26.652 --> 21:28.788 When they started to turn black,we started to get 21:28.788 --> 21:32.424 very concerned, not knowingif we had done something wrong, 21:32.424 --> 21:35.094 or not knowing if we hadpotentially killed 21:35.094 --> 21:37.930 all these sponge fragmentsthat we had harvested. 21:37.930 --> 21:41.400 However, we were very relievedto see that, even the next day, 21:41.400 --> 21:44.236 the blackness goes away,the sponges are doing great, 21:44.236 --> 21:46.639 and they appear to bereattaching very quickly 21:46.639 --> 21:49.241 to both the rubble piecesthat they've been attached 21:49.241 --> 21:53.746 to and also to the grow outstructures. 21:53.746 --> 21:56.348 >>NARRATOR: For 40 days,Martin Moe has been monitoring 21:56.348 --> 22:00.152 the survival and growthof the Diadema larvae. 22:00.152 --> 22:02.621 A week ago, he hit a setback-- 22:02.621 --> 22:06.492 all three cultures were struckby an infestation of Vorticella, 22:06.492 --> 22:10.429 an organism that causescompetition for food 22:10.429 --> 22:14.767 and a declinein the larval development. 22:14.767 --> 22:17.336 But one of the cultures showeda larger number 22:17.336 --> 22:20.840 of well-formed larvaethan the other two. 22:20.840 --> 22:24.276 He made a decision to dedicatethe remaining food supply 22:24.276 --> 22:26.712 to the survivalof the well-formed culture 22:26.712 --> 22:29.782 and abandon the others. 22:29.782 --> 22:33.085 It might be the only chancefor at least some of the larvae 22:33.085 --> 22:36.255 to reach the settlement phase. 22:36.255 --> 22:38.324 >>Now, this one is readyto come out, 22:38.324 --> 22:42.261 and we can check for settlerson it. 22:42.261 --> 22:46.232 And they settle as larvae,and then they go 22:46.232 --> 22:50.169 through metamorphosis in whichthe spines and larval tissue 22:50.169 --> 22:53.038 all drop down into the rudiment,and the rudiment becomes 22:53.038 --> 22:55.808 a little round juvenile. 22:55.808 --> 22:58.310 And you can pull it outof the settlement, like this, 22:58.310 --> 23:01.413 and the water staysin these little cubicles. 23:01.413 --> 23:07.553 Then, you put it right downinto the settlement tray. 23:07.553 --> 23:10.489 The water in the settlement trayis all adjusted for temperature, 23:10.489 --> 23:14.994 pH, alkalinity, calcium,and it's ready to support 23:14.994 --> 23:20.566 the process of metamorphosisand the early juvenile. 23:23.235 --> 23:25.070 After they go throughmetamorphosis, 23:25.070 --> 23:28.207 they're a soft little creature. 23:28.207 --> 23:31.110 And they can't surviveas a soft little creature. 23:31.110 --> 23:33.412 They have to pullcalcium carbonate, 23:33.412 --> 23:37.349 the same stuff your bones aremade out of, out of the water, 23:37.349 --> 23:40.386 which is dissolved in the water,and they have to form 23:40.386 --> 23:43.322 their hard parts andtheir mouth parts so they can 23:43.322 --> 23:48.427 actually scrape and feedand survive as a sea urchin, 23:48.427 --> 23:52.064 rather than a pelagicfloating larvae. 23:52.064 --> 23:54.934 And then, it can be carriedand placed down 23:54.934 --> 23:59.004 into this racewaykind of facility. 23:59.004 --> 24:00.272 I call it a raceway. 24:00.272 --> 24:03.108 There's a water currentthat moves all through 24:03.108 --> 24:06.745 the whole tank like this,and as they are settling there, 24:06.745 --> 24:10.215 it keeps the water freshand clear around them. 24:10.215 --> 24:16.155 They grow and become littleurchins on these plates. 24:16.155 --> 24:18.924 At some point, it becomesoptimum to be able 24:18.924 --> 24:21.927 to pick the plate upand put it in a bucket. 24:21.927 --> 24:25.130 They'll stay stuck on the plate,and then you can move them 24:25.130 --> 24:30.269 to any kind of a grow-out areathat you wish. 24:30.269 --> 24:33.672 >>NARRATOR: After four yearsof developing the technology 24:33.672 --> 24:36.041 to raise lab-cultured Diadema, 24:36.041 --> 24:40.346 Martin Moe is nearing the endof his quest. 24:40.346 --> 24:43.015 >>When we get to the pointwhere we can produce 24:43.015 --> 24:46.418 large numbersof juvenile Diadema, 24:46.418 --> 24:50.055 then we can begin to researchon what is the best way 24:50.055 --> 24:52.992 to reestablish themon the reef. 24:52.992 --> 24:55.627 At that point, along withKen's work with the corals 24:55.627 --> 24:59.498 and what I've been ableto develop with the Diadema, 24:59.498 --> 25:03.435 we can come together and we canachieve at least some semblance 25:03.435 --> 25:06.438 of ecological restorationon the reefs. 25:12.011 --> 25:13.178 >>NARRATOR:At the grounding site, 25:13.178 --> 25:17.416 the sponge transplantsare healthy and growing. 25:17.416 --> 25:20.719 Amanda Bourque is watchfulas the sponges replicate 25:20.719 --> 25:25.224 their natural behaviorin a transformed environment. 25:25.224 --> 25:28.927 But it will take several yearsof monitoring before the success 25:28.927 --> 25:32.097 of this project is fully known. 25:36.368 --> 25:40.339 Coral reef restorationis an emerging science 25:40.339 --> 25:44.376 built on innovative ideas,lessons learned, 25:44.376 --> 25:48.447 and continual refinementof the human technologies 25:48.447 --> 25:54.453 that are helping nature restoreits gardens of the sea. 26:24.850 --> 26:27.286 >>Major funding for thisprogram was provided 26:27.286 --> 26:29.288 by the Batchelor Foundation, 26:29.288 --> 26:32.091 encouraging peopleto preserve and protect 26:32.091 --> 26:36.091 America's underwater resources.